(1)
🔸️ Next Generation Sequencing (NGS)
🔹️ A powerful platform that sequence millions of DNA molecule simultaneously.
🔹️Types of NGS
• Sequencing during synthesis
- pyrosequencing
- illumina
- Ion torrent
• Direct Sequencing
-Nanopore Sequencing
🔸️ Next Generation Sequencing (NGS)
🔹️ A powerful platform that sequence millions of DNA molecule simultaneously.
🔹️Types of NGS
• Sequencing during synthesis
- pyrosequencing
- illumina
- Ion torrent
• Direct Sequencing
-Nanopore Sequencing
(2)
• Sequencing during synthesis
- pyrosequencing :
The concept; is to detect the light radiate from pyrophosphate that is released from polymerization process.
- Beads as a soild surface attached to it pices of DNA are needed.
- Templet DNA fragments with adaptor DNA.
• Sequencing during synthesis
- pyrosequencing :
The concept; is to detect the light radiate from pyrophosphate that is released from polymerization process.
- Beads as a soild surface attached to it pices of DNA are needed.
- Templet DNA fragments with adaptor DNA.
(3)
- 454 Sequencing
Explains the process from start to end using wells plate with 2 million wells. With the help of CCD( high resolution charge coupled device camera) the light will be captured.
NOTE:
The whole process will be conducted for one dNTP type each time.
- 454 Sequencing
Explains the process from start to end using wells plate with 2 million wells. With the help of CCD( high resolution charge coupled device camera) the light will be captured.
NOTE:
The whole process will be conducted for one dNTP type each time.
(4)
-illumina
To detect the fluorescent labeled dNTPs by passing laser beam.
4 main steps:
1\ sample preparation
-Adaptor region
2\ cluster generation
- solid surface; chip
- Bridge Building
- Bridge amplification
3\sequencing
- fluorescent labeled dNTPs
4\ Data analysis
-illumina
To detect the fluorescent labeled dNTPs by passing laser beam.
4 main steps:
1\ sample preparation
-Adaptor region
2\ cluster generation
- solid surface; chip
- Bridge Building
- Bridge amplification
3\sequencing
- fluorescent labeled dNTPs
4\ Data analysis
(5)
- Ion torrent sequencing
* We will continue later* 😁
- Ion torrent sequencing
* We will continue later* 😁
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