Hello everyone,
In this flash talk (thread) for #Popgroup55, happy to present: "Genotyping analysis and frequency of kdr mutations in Saudi Arabian Aedes aegypti: Evolution of resistance"
#SaudiArabia
#Aedes_aegypti
Sponsor: @uksacborg & @JazanUniversity
#pgg55 #pggflash
In this flash talk (thread) for #Popgroup55, happy to present: "Genotyping analysis and frequency of kdr mutations in Saudi Arabian Aedes aegypti: Evolution of resistance"
#SaudiArabia
#Aedes_aegypti
Sponsor: @uksacborg & @JazanUniversity
#pgg55 #pggflash
Intro
Aedes aegypti mosquito is responsible for the transmission of dengue fever. Controlling Ae. aegypti relies on insecticides; however, mosquitoes have evolved resistance worldwide. We aim to characterize Vssc mutations (Fig1) and haplotypes in Saudi Arabia and SE Asia.
Aedes aegypti mosquito is responsible for the transmission of dengue fever. Controlling Ae. aegypti relies on insecticides; however, mosquitoes have evolved resistance worldwide. We aim to characterize Vssc mutations (Fig1) and haplotypes in Saudi Arabia and SE Asia.
Methodology
Ae. aegypti were collected from five regions in Saudi A.(Fig2). DNA was extracted and assayed for known kdr mutations in domains IIS6&IIIS6(Fig3) using PCR and sequencing (Fig4). PopART software was used to construct a putative evolutionary link amongst the sequences.
Ae. aegypti were collected from five regions in Saudi A.(Fig2). DNA was extracted and assayed for known kdr mutations in domains IIS6&IIIS6(Fig3) using PCR and sequencing (Fig4). PopART software was used to construct a putative evolutionary link amongst the sequences.
Results-1
Allele frequencies are shown in (Table1) and (Fig5). The S989P mutation was in perfect linkage with V1016G, as reported previously. The allele frequency varied between regions in Saudi A.; however, the F1534C mutation seems widely distributed in Jazan and Sahil.
Allele frequencies are shown in (Table1) and (Fig5). The S989P mutation was in perfect linkage with V1016G, as reported previously. The allele frequency varied between regions in Saudi A.; however, the F1534C mutation seems widely distributed in Jazan and Sahil.
Results-2
Interestingly, we detected a dozen individuals with a genotype implying the presence of haplotypes containing no kdr mutations and one individual with triple homozygous mutant. Of the 9 genotypes, the majority were homozygous mutant and heterozygous for F1534C (Fig6).
Interestingly, we detected a dozen individuals with a genotype implying the presence of haplotypes containing no kdr mutations and one individual with triple homozygous mutant. Of the 9 genotypes, the majority were homozygous mutant and heterozygous for F1534C (Fig6).
Results-3
The analysis of our sequences and GenBank from 22 countries revealed 6 haplotypes and 26 haplotypes, respectively, for the IIS6 and IIIS6 segments (Fig7A&B). There are some haplotypes spread out from the original population of Africa (Fig7 B) to the rest of the world.
The analysis of our sequences and GenBank from 22 countries revealed 6 haplotypes and 26 haplotypes, respectively, for the IIS6 and IIIS6 segments (Fig7A&B). There are some haplotypes spread out from the original population of Africa (Fig7 B) to the rest of the world.
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