There is something fishy about the monkey pox sequences.
50 mutations is too many for a 2018 DNA virus.
There is also a deletion which hints at serial passaging.
virological.org
50 mutations is too many for a 2018 DNA virus.
There is also a deletion which hints at serial passaging.
virological.org
Serial passaging can result in deletions in pox viruses.
Depending on the host cell line, you get different APOBEC activity.
Depending on the host cell line, you get different APOBEC activity.
As for concern over WEF like agendas.
Itās still really early to make definitive statements about leaks Vs zoonosis.
While statists love to run simulations where they salivate over SimCity like god gamesā¦ they need an exaggerated sim to feed interventionist ego.
C19 fell short
Itās still really early to make definitive statements about leaks Vs zoonosis.
While statists love to run simulations where they salivate over SimCity like god gamesā¦ they need an exaggerated sim to feed interventionist ego.
C19 fell short
Last week I was holding out for confirmatory sequence data from Illumina. This is important as ONT nanopore can have insertion/deletion error and also higher error rate around methylated bp. Illumina sequence is now in confirming the sequence.
Some links to relevant citations
Some links to relevant citations
Some details about APOBEC3.
Some references to the sequencing methods.
Important citation.
virological.org
virological.org
From isidro et al.
Important to highlight that the reads are not yet public but they should be this week.
Molnupiravir does create C->T mutations but as you can see from the two OH groups on the base, this is an RNA base and shouldnāt get incorporated into a DNA virus at high frequency.
It also doesnāt have dinucleotide and tetranucleotide sequence preferences like APOBEC.
It also doesnāt have dinucleotide and tetranucleotide sequence preferences like APOBEC.
Itās mutation rate in SARs is reckless in my opinion. You donāt add mutagens mid pandemic after you have compromised peopleās immune systems with the jab.
ncbi.nlm.nih.gov
ncbi.nlm.nih.gov
A few things about APOBEC.
We have used this enzyme to study methylation patterns in Cannabis
Itās available from NEB. So in theory a DNA virus could be treated with this and transfected to get mutagenesis on a DNA virus but itās not a very directed edit
biorxiv.org
We have used this enzyme to study methylation patterns in Cannabis
Itās available from NEB. So in theory a DNA virus could be treated with this and transfected to get mutagenesis on a DNA virus but itās not a very directed edit
biorxiv.org
Iām struggling to see why someone who do this when there are more directed ways they can edit viruses in 2018. Perhaps as a smoke screen to obscure origins but thatās getting into some Tin hat space.
It may be a side effect of cell culture.
There are other people questions.
It may be a side effect of cell culture.
There are other people questions.
APOBECs require zinc. Many populations are consuming more zinc.
APOBECs are also up-regulated with LINE1 expression. Vax and C19 could be inducing more LINE1 expression.
APOBEC has left noticeable footprints on human/C19 evolution.
ncbi.nlm.nih.gov
APOBECs are also up-regulated with LINE1 expression. Vax and C19 could be inducing more LINE1 expression.
APOBEC has left noticeable footprints on human/C19 evolution.
ncbi.nlm.nih.gov
I have questions regarding what pseudouridine does to APOBEC biology. Injecting large portions of the population with lots of m1pU could alter the expression of these enzymes.
mdpi.com
mdpi.com
While there are many ānaturalā or c19/vax induced hypothesis one could imagine hyper-expressing APOBEC activity, I would expect to see some intermediate mutations.
0 to 50 mutations isnāt natural unless we failed to sequence the intermediates.
0 to 50 mutations isnāt natural unless we failed to sequence the intermediates.
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