Alex Washburne
Alex Washburne

@WashburneAlex

5 Tweets 6 reads Dec 10, 2023
It's been over a years since our paper was released.
We're proud to say it has withstood every criticism we've heard, and we've kept our ears open this whole time.
SARS-CoV-2 is unusual among wild coronaviruses in exactly the way it is consistent with a synthetic virus.
I want to extend a special thank-you to the amazing collaborators @VBruttel and @tony_vandongen whose bioengineering expertise was an excellent synergy with my quantitative & computational biology.
Also shout-out to @quay_dr who uncovered & examined this issue independently.
One connection we didn't mention in 2022:
The only time these two enzymes - BsaI + BsmBI - were used in a coronavirus pre-COVID was in a 2017 paper with Ben Hu, Peter Daszak, Shi ZhengLi et al, acknowledging NIH support.
A key piece of the puzzle.
journals.plos.org
When I worked on pathogen spillover pre-COVID as part of a DARPA PREEMPT team, I was examining the origins & evolution of viruses, assessing likely sources, and more.
Daszak et al. proposed DEFUSE to DARPA's PREEMPT call aimed to synthesize a bat SARS CoV like SARS2.
It's a bit ironic, and perhaps redemptive, that a former DARPA PREEMPT researcher should help uncover the likely role of DEFUSE collaborators in the creation of SARS-CoV-2.
Needless to say, it has cost me all my connections in the field to do so.

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